Recombinant Mouse Factor H/CFH Protein

來源: 發(fā)布時間:2024-05-16

牛纖維蛋白原(Bovine Fibrinogen, BFg)作為一種多功能的凝血蛋白,在血液凝固和傷口愈合過程中扮演著關鍵角色。本綜述旨在探討牛纖維蛋白原的分子結(jié)構(gòu)、生物學功能以及其在生物醫(yī)學研究中的潛在應用。引言纖維蛋白原是血液中的一種重要糖蛋白,它參與血液凝固的階段,通過轉(zhuǎn)化為纖維蛋白來形成穩(wěn)定的血栓。牛纖維蛋白原因其與人類纖維蛋白原的高同源性,常被用作研究模型,以深入理解纖維蛋白原的功能及其在疾病中的作用。材料與方法蛋白提取與純化:從牛血中提取纖維蛋白原,并通過多次沉淀和層析技術(shù)進行純化。分子結(jié)構(gòu)分析:利用質(zhì)譜、X射線晶體學等技術(shù)解析牛纖維蛋白原的分子結(jié)構(gòu)。功能研究:通過體外實驗和動物模型研究牛纖維蛋白原在血液凝固和細胞黏附中的作用。CX3CL1受到促炎性刺激的上調(diào),包括巨噬細胞,樹突狀細胞,內(nèi)皮細胞,神經(jīng)元,平滑肌,上皮細胞的細胞類型。Recombinant Mouse Factor H/CFH Protein,His Tag

Recombinant Mouse Factor H/CFH Protein,His Tag,標準物質(zhì)

RecombinantBiotinylatedCynomolgusSiglec-10Protein,His-AviTag性能參數(shù)分子別名(Synonyms)SLG2;SIGLEC10;MGC126774;PRO940表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedCynomolgusSiglec-10ProteinisexpressedfromHEK293withHistagandAvitagattheC-terminus.ItcontainsThr17-Asn552.[Accession|A0A2K5WBX8]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof61.71kDa.Duetoglycosylation,theproteinmigratesto72-82kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGE制劑(Formulation)Suppliedas0.22μmfilteredsolutionin25mMMES,150mMNaCl,0.5MArginine(pH5.0).儲存條件Theproductshouldbestoredat-85~-65℃for1yearfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.Recombinant Human LAG3/CD223 Protein,hFc Tag成熟的人IL-28A是一種約22-25 kDa蛋白,與小鼠和大鼠IL-28A共享66%的氨基酸序列身份,并顯示跨物種活性。

Recombinant Mouse Factor H/CFH Protein,His Tag,標準物質(zhì)

性能參數(shù)表達區(qū)間及表達系統(tǒng)(Source)CynomolgusCD27Ligand/CD70ProteinisexpressedfromHEK293withHistagattheN-terminal.ItcontainsGln39-Pro194.[Accession|G7PYU6-1]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof18.4kDa.Duetoglycosylation,theproteinmigratesto60-90kDabasedonTris-BisPAGEresult.(Endotoxin)Lessthan1EUperugbytheLALmethod.純度(Purity)>95%asdeterminedbyTris-BisPAGE活性(Activity)ELISAData:ImmobilizedCynomolgusCD27Ligand,HisTagat2μg/ml(100μl/well)ontheplate.DoseresponsecurveforCynomolgus/RhesusmacaqueCD27,hFcTagwiththeEC50of70.8ng/mldeterminedbyELISA.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構(gòu)方法(Reconstitution)Centrifugetubesbeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.

IdeSProtease全稱免疫球蛋白G降解酶,酶活(Enzymeactivity)40U/μL酶活定義(UnitDefinition)在37℃條件下,反應30min,剪切>95%的1μg重組單克隆IgG所需要的酶量定義為一個活性單位。儲存條件-25~-15℃保存,有效期1年。使用方法1.在消化液中加入適量IgG(加至5mg);2.在IgG樣本中加入IdeS蛋白酶(每1μgIgG加入1個單位的IdeS);3.將樣品置于37℃孵育30-60min。IdeS蛋白酶在中性pH或接近中性pH的緩沖中活性強。推薦反應緩沖是50mM磷酸鈉,150mMNaCl(pH6.6),多數(shù)常見的生物緩沖也適用,比如Tris或PBS;注意:不在此pH范圍(例如乙酸鹽緩沖液)的緩沖也可能適用,但是孵育時間或酶量需要根據(jù)實際情況進行優(yōu)化。注意事項1.IdeS不能識別切割小鼠IgG1/IgG2b,大鼠、豬、牛和山羊IgG,對小鼠IgG2a和IgG3具有中等酶切活性,酶切小鼠IgG2a和IgG3建議增加IdeS的用量(推薦用量為正常用量的5-10倍)。2.IdeS不能切割非IgG亞型的單抗分子,包括IgA、IgM、IgD和IgE。FGF-18與FGF R2C,F(xiàn)GF R3C以及高爾基蛋白GLG1結(jié)合,并誘導星形膠質(zhì)細胞和小膠質(zhì)細胞。

Recombinant Mouse Factor H/CFH Protein,His Tag,標準物質(zhì)

RecombinantBiotinylatedHumanKIR2DL1Protein,His-AviTag性能參數(shù)分子別名(Synonyms)CD158A;CD158Ankat1;cl-42表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedHumanKIR2DL1ProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsHis22-Arg242.[Accession|P43626]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof27.1kDa.Duetoglycosylation,theproteinmigratesto48-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構(gòu)方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.儲存條件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.透明質(zhì)酸(Hyaluronic Acid,簡稱HA)是一種存在于人體和動物組織中的天然高分子多糖,稱為糖醛酸或玻尿酸。Recombinant Human BAFFR/TNFRSF13C Protein,hFc Tag

AFGF在調(diào)節(jié)細胞存活,細胞分裂,血管生成,細胞分化和細胞遷移中起重要作用。Recombinant Mouse Factor H/CFH Protein,His Tag

Name:AITRL,MouseSynonyms:Activation-inducedTNFRmemberLigand,TNFSF18,GITRL,TL-6Description:Activation-InducibleTNF-RelatedLigand(AITRL),alsoknownasGlucocorticoid-InducedTNF-RelatedLigand(GITRL),belongstothetumornecrosisfactorsuperfamily(TNFSF).AITRLisaTypeIIsingletransmembraneproteinandshareslowconservationwithintheextracellulardomainwithotherTNFSFmembers.AITRLisexpressedonmacrophages,immatureandmaturedendriticcellsandBcells.Itsreceptor,Activation-InducibleTNFRfamilyReceptor(AITR),isexpressedonTlymphocytes,naturalkiller(NK)cells,andantigen-presentingcells.AfterbindingbyAITRL,AITRcanbereleased.AITRactivationincreasesresistancetotumorsandviralinfectionsandisinvolvedinautoimmuneandinflammatoryprocesses.Inaddition,activatedAITRincreasesTCR-inducedTcellproliferationandcytokineproductionandrescuesTcellsandNKcellsfromapoptosis.RecombinantmouseActivation-InducibleTNF-RelatedLigand(rmAITRL)producedinE.Recombinant Mouse Factor H/CFH Protein,His Tag